Composite

Part:BBa_K3831030

Designed by: Klára Odehnalová   Group: iGEM21_Brno_Czech_Republic   (2021-10-07)


Construct A Construct A for production of BMC

This composite part was designed to be used to test and characterise the production and assembly of bacterial microcompartments BMCs in Bacillus subtilis. Its main components are the five shell proteins of Pdu BMC from bacteria Parageobacilus thermoglucosidasius. These genes were placed under a promoter inducible by IPTG for the purpose of verifying their production in B. subtilis. Additionally, this construct was designed in a way that would allow for the BMC genes and their respective ribosomal binding sites to be cleaved out by restriction enzymes and subsequently ligated to the alteret Pgrac promoter in order to assemble composite part Z.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 250
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2843
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1108
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds
//chassis/prokaryote/bsubtilis
//function/structures
Parameters
chassisBacillus subtilis